Anti-Notch1 antibody [mN1A] (ab280898)
![Anti-Notch1 antibody [mN1A] (ab280898)](https://www.abcam.com/ps/products/280/ab280898/Images/ab280898-459730-anti-notch1-antibody-mn1-western-blot-wild-type-hap11.jpg "Anti-Notch1 antibody [mN1A] (ab280898)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [mN1A] to Notch1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Notch1 antibody [mN1A]
See all Notch1 primary antibodies -
Description
Mouse monoclonal [mN1A] to Notch1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- WB: Wild type HAP1 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
mN1A -
Isotype
IgG1
Images
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Western blot - Anti-Notch1 antibody [mN1A] (ab280898)All lanes : Anti-Notch1 antibody [mN1A] (ab280898) at 1/1000 dilution
Lane 1 : Wild type HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
Lane 2 : NOTCH1 knockout HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution
Predicted band size: 272 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lanes 1-2: Merged signal (red and green). Green - ab39062 observed at 125 kDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab39062 Anti-NOTCH1 antibody was shown to react with NOTCH1 in HAP1 cells in Western blot. Loss of signal was observed when NOTCH1 knockout sample was used. Wild-type and NOTCH1 knockout samples were subjected to SDS-PAGE.
ab39062 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.