Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23813-37] to NOR1/TEC - BSA and Azide free
  • Suitable for: WB, IP, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free
    See all NOR1/TEC primary antibodies

  • Description

    Rabbit monoclonal [EPR23813-37] to NOR1/TEC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt (Intra) or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, MCF7, MDA-MB-231 lysates. ICC: HeLa cells. IP: HeLa cells.

  • General notes

    ab281833 is the carrier-free version of ab259939. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281833 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23813-37

  • Isotype

    IgG

Images

  • Western blot - Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)
    Western blot - Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)
    All lanes : Anti-NOR1/TEC antibody [EPR23813-37] (ab259939) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : MDA-MB-231 (human breast adenocarcinoma epithelial cell)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
    Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 68 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    This data was developed using ab259939, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 125 seconds.

     

  • Immunocytochemistry - Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)
    Immunocytochemistry - Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)

    This data was developed using ab259939, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling NOR1/TEC with ab259939 at 1/50 (10.16 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)
    Immunoprecipitation - Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)

    This data was developed using ab259939, the same antibody clone in a different buffer formulation.

    NOR1/TEC was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug with ab259939 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259939 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab259939 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259939 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)
    Anti-NOR1/TEC antibody [EPR23813-37] - BSA and Azide free (ab281833)

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