Anti-NOD1 antibody [EPR20833] (ab215726)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20833] to NOD1
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-NOD1 antibody [EPR20833]
See all NOD1 primary antibodies -
Description
Rabbit monoclonal [EPR20833] to NOD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type BMDM whole cell lysate; mouse lung tissue lysate; mouse spleen tissue lysate.
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General notes
This product was previously labelled as CARD4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20833 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution
Lane 1 : Mouse lung lysate boiled before loading
Lane 2 : Mouse lung lysate not boiled before loading
Lane 3 : Mouse spleen lysate boiled before loading
Lane 4 : Mouse spleen lysate not boiled before loading
Lane 5 : Mouse heart lysate boiled before loading
Lane 6 : Mouse heart lysate not boiled before loading
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe 100-kDa band represents NOD1 monomer and the band above 250 kDa is the aggregates. Other bands are unspecific.
This antibody detects a strong BOI (band of interest) in mouse lung and mouse spleen, but a weak BOI in mouse heart which also expresses NOD1 (PMID:25786158, PMID:28878001, PMID:23028889).
Loading control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
Blocking buffer: 5% NFDM/TBST
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Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution + Mouse lung tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
NOD1 is an alternative name for CARD4.
The 100-kDa band is the specific NOD1 band, other bands are unspecific.
The lysates were un-boiled to avoid the protein aggregation.
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All lanes : Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution
Lane 1 : Wild-type BMDM (mouse bone marrow-derived macrophages), whole cell lysate
Lane 2 : NOD1 knockout BMDMs, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST
The 100-kDa band is the specific NOD1 band, other bands are unspecific.
NOD1 is an alternative name for CARD4.
The WT and NOD1 KO BMDMs lysates were kindly provided by our collaborator Dr. Gang Liu. Tsinghua University. -