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Anti-NOD1 antibody [EPR20833] (ab215726)

Price and availability

314 937 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-NOD1 antibody [EPR20833] (ab215726)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20833] to NOD1
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-NOD1 antibody [EPR20833]
    See all NOD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20833] to NOD1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type BMDM whole cell lysate; mouse lung tissue lysate; mouse spleen tissue lysate.
  • General notes

     This product was previously labelled as CARD4

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20833
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family

Images

  • Western blot - Anti-NOD1 antibody [EPR20833] (ab215726)
    Western blot - Anti-NOD1 antibody [EPR20833] (ab215726)
    All lanes : Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution

    Lane 1 : Mouse lung lysate boiled before loading
    Lane 2 : Mouse lung lysate not boiled before loading
    Lane 3 : Mouse spleen lysate boiled before loading
    Lane 4 : Mouse spleen lysate not boiled before loading
    Lane 5 : Mouse heart lysate boiled before loading
    Lane 6 : Mouse heart lysate not boiled before loading

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The 100-kDa band represents NOD1 monomer and the band above 250 kDa is the aggregates. Other bands are unspecific.

    This antibody detects a strong BOI (band of interest) in mouse lung and mouse spleen, but a weak BOI in mouse heart which also expresses NOD1 (PMID:25786158, PMID:28878001, PMID:23028889).

    Loading control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

    Blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-NOD1 antibody [EPR20833] (ab215726)
    Western blot - Anti-NOD1 antibody [EPR20833] (ab215726)
    Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution + Mouse lung tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    NOD1 is an alternative name for CARD4.

    The 100-kDa band is the specific NOD1 band, other bands are unspecific.

    The lysates were un-boiled to avoid the protein aggregation.

  • Western blot - Anti-NOD1 antibody [EPR20833] (ab215726)
    Western blot - Anti-NOD1 antibody [EPR20833] (ab215726)
    All lanes : Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution

    Lane 1 : Wild-type BMDM (mouse bone marrow-derived macrophages), whole cell lysate
    Lane 2 : NOD1 knockout BMDMs, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    The 100-kDa band is the specific NOD1 band, other bands are unspecific.

    NOD1 is an alternative name for CARD4.

    The WT and NOD1 KO BMDMs lysates were kindly provided by our collaborator Dr. Gang Liu. Tsinghua University.

  • Anti-NOD1 antibody [EPR20833] (ab215726)
    Anti-NOD1 antibody [EPR20833] (ab215726)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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