Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24351-6] to nNOS (neuronal) - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free
    See all nNOS (neuronal) primary antibodies

  • Description

    Rabbit monoclonal [EPR24351-6] to nNOS (neuronal) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra),ICC,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human thalamus and cerebellum tissue.

  • General notes

    ab281832 is the carrier-free version of ab270958. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281832 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24351-6

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)

    This data was developed using ab270958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human thalamus tissue labelling nNOS (neuronal) with ab270958 at 1/200 (2.33 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab270958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Cytoplasmic staining on some neurons in human thalamus.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)

    This data was developed using ab270958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labelling nNOS (neuronal) with ab270958 at 1/200 (2.33 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab270958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Positive staining in human cerebellum.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)

    This data was developed using ab270958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human liver tissue labelling nNOS (neuronal) with ab270958 at 1/200 (2.33 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab270958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human liver (PMID: 8626668).

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)

    This data was developed using ab270958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling nNOS (neuronal) with ab270958 at 1/200 (2.33 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab270958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human cardiac muscle (PMID: 8626668).

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)
    Anti-nNOS (neuronal) antibody [EPR24351-6] - BSA and Azide free (ab281832)

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