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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes ADP-ribosylation

Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)

Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI1F7] to Nmnat1/NMNAT
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Nmnat1/NMNAT antibody [OTI1F7]
    See all Nmnat1/NMNAT primary antibodies
  • Description

    Mouse monoclonal [OTI1F7] to Nmnat1/NMNAT
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, African green monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Nmnat1/NMNAT aa 1 to the C-terminus. Produced in HEK-293T cells (NP_073624).
    Database link: Q9HAN9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Nmnat1/NMNAT transfected HEK-293T cell lysate. ICC/IF: COS-7 cells transiently transfected with Nmnat1/NMNAT.
  • General notes

    Clone OTI1F7 (formerly 1F7).

     This product was previously labelled as Nmnat1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI1F7
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • ADP-ribosylation
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)
    Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)
    All lanes : Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)
    Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)

    pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Nmnat1/NMNAT using ab118270 at a 1/100 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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