Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)
![Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)](https://www.abcam.com/ps/products/174/ab174636/Images/ab174636-311603-anti-nmdar2a-antibody-n327a-38-n-terminal-immunofluorescence.jpg "Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)")
Key features and details
- Mouse monoclonal [N327A/38] to NMDAR2A - N-terminal
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-NMDAR2A antibody [N327A/38] - N-terminal
See all NMDAR2A primary antibodies -
Description
Mouse monoclonal [N327A/38] to NMDAR2A - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment (His-tag) within Rat NMDAR2A aa 50-350 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00959 -
Epitope
extracellular domain -
Positive control
- COS cells transiently transfected with GFP-tagged NMDAR2A lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S327A-38 to N327A/38, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N327A/38 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)
4% formaldehyde-fixed SK-N-BE cells labeling NMDAR2A using ab174636 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain and DAPI (blue) nuclear stain.
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![Western blot - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)](https://www.abcam.com/ps/products/174/ab174636/Images/ab174636-316906-anti-nmdar2a-antibody-n327a-38-n-terminal-western-blot.jpg)
Western blot - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636) at 1/200 dilution + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg with Monkey COS cells transfected with GFP-tagged NR2A
Secondary
Goat Anti-Mouse IgG: HRP for 1 hour RT. at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: ~170 kDa why is the actual band size different from the predicted?Color Development: ECL solution for 6 min in RT.
