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Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)

Key features and details

  • Mouse monoclonal [N327A/38] to NMDAR2A - N-terminal
  • Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-NMDAR2A antibody [N327A/38] - N-terminal
    See all NMDAR2A primary antibodies

  • Description

    Mouse monoclonal [N327A/38] to NMDAR2A - N-terminal

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Recombinant fragment (His-tag) within Rat NMDAR2A aa 50-350 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q00959

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    extracellular domain

  • Positive control

    • COS cells transiently transfected with GFP-tagged NMDAR2A lysate. ICC/IF: SK-N-BE cells.

  • General notes

    The clone number has been updated from S327A-38 to N327A/38, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)
    Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)

    4% formaldehyde-fixed SK-N-BE cells labeling NMDAR2A using ab174636 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain and DAPI (blue) nuclear stain.

  • Western blot - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)
    Western blot - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636)
    Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636) at 1/200 dilution + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg with Monkey COS cells transfected with GFP-tagged NR2A

    Secondary
    Goat Anti-Mouse IgG: HRP for 1 hour RT. at 1/1000 dilution

    Predicted band size: 165 kDa
    Observed band size: ~170 kDa
    why is the actual band size different from the predicted?



    Color Development: ECL solution for 6 min in RT.

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