Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NLRP3 antibody [EPR23726-24] - BSA and Azide free (ab273586)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23726-24] to NLRP3 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NLRP3 antibody [EPR23726-24] - BSA and Azide free
    See all NLRP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23726-24] to NLRP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 and THP-1 (treated with 50 nM PMA and 5 ug/ml LPS(Lipopolysaccharide) for 24h), whole cell lysates. IP: THP-1 whole cell lysate.

  • General notes

    ab273586 is the carrier-free version of ab260017. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-NLRP3 antibody [EPR23726-24] - BSA and Azide free (ab273586)
    Immunoprecipitation - Anti-NLRP3 antibody [EPR23726-24] - BSA and Azide free (ab273586)

    NLRP3 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab260017 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260017 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate 10 ug

    Lane 2: ab260017 IP in THP-1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260017 in THP-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260017).

  • Anti-NLRP3 antibody [EPR23726-24] - BSA and Azide free (ab273586)
    Anti-NLRP3 antibody [EPR23726-24] - BSA and Azide free (ab273586)

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