Anti-NLN antibody [OTI1D6] (ab119802)
![Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-1.jpg "Anti-NLN antibody [OTI1D6] (ab119802)")
Key features and details
- Mouse monoclonal [OTI1D6] to NLN
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-NLN antibody [OTI1D6]
See all NLN primary antibodies -
Description
Mouse monoclonal [OTI1D6] to NLN -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human NLN aa 1-750. Produced in HEK293T cells (NP_065777).
Database link: Q9BYT8 -
Positive control
- WB: HEK-293T cells transfected with NLN, HepG2, HeLa, SVT2, A549, COS7, PC12, and MCF7 cell extracts. IHC-P: Carcinoma of Human pancreas, Adenocarcinoma of Human endometrium, Carcinoma of Human bladder and Human tonsil tissues. ICC/IF: COS7 cells transiently transfected with NLN. Flow: Jurkat cells.
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General notes
The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI1D6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-NLN antibody [OTI1D6] (ab119802)All lanes : Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NLN cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 81 kDa
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![Western blot - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-2.jpg)
Western blot - Anti-NLN antibody [OTI1D6] (ab119802)All lanes : Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : MDCK cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line)
cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 81 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human pancreas carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human endometrium adenocarcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human bladder carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (ab119802)Paraffin-embedded human tonsil tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/NLN-Primary-antibodies-ab119802-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (ab119802)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY NLN, stained for NLN (green) using ab119802 at 1/100 dilution in ICC/IF.
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![Flow Cytometry - Anti-NLN antibody [OTI1D6] (ab119802)](https://www.abcam.com/ps/products/119/ab119802/Images/ab119802-314133-143.jpg)
Flow Cytometry - Anti-NLN antibody [OTI1D6] (ab119802)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab119802 at 1/100 dilution stained for NLN (Red) compared to a nonspecific negetive control antibody (Blue).
