Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16653] to Nkx3.1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free
    See all Nkx3.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16653] to Nkx3.1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251217 is the carrier-free version of ab196020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251217 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    All lanes : Anti-Nkx3.1 antibody [EPR16653] (ab196020) at 1/1000 dilution

    Lane 1 : LNCaP (Human prostate cancer cell line) cell lysate
    Lane 2 : PC-3 (Human prostate cancer cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab196020, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Lane 2, PC-3 (Human prostate cancer cell line) is acting as a negative control for Nkx3.1. The 32kDa band may represent the glycosylated form.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    This data was developed using ab196020, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling Nkx3.1 with ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human prostate hyperplasia tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    Anti-Nkx3.1 antibody [EPR16653] (ab196020) at 1/1000 dilution + Human prostate lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab196020, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    This data was developed using ab196020, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Nkx3.1 with ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human testis tissue acting as a negative control for Nkx3.1. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
    Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)

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