Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
![Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)](https://www.abcam.com/ps/products/228/ab228335/Images/ab228335-291686-anti-nkx25-antibody-epr20168-western-blot.jpg "Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free
See all Nkx2.5 primary antibodies -
Description
Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart muscle tissue lysate; HEK-293T and HeLa whole cell lysate.
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General notes
Ab228335 is the carrier-free version of ab205263. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab228335 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20168 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution
Lane 1 : Human fetal heart muscle tissue lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 30-42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution: 5% NFDM/TBST
The molecular masses observed are consistent with what has been described in the literature (PMID: 21447557)
Negative control: MCF7 (PMID: 15340050).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205263).
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![Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)](https://www.abcam.com/ps/products/228/ab228335/Images/ab228335-2-benefits-of-recombinant-antibodies.png)
Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
