Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-NKp30 antibody [EPR14509] (ab186425)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14509] to NKp30
Suitable for: IP, WB
Reacts with: Rat, Human

Product name

Anti-NKp30 antibody [EPR14509]
See all NKp30 primary antibodies
Description

Rabbit monoclonal [EPR14509] to NKp30
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- THP1, NCI-H460, K562 and rat brain lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14509
Isotype

IgG
Research areas

Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)

All lanes : Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution

Lane 1 : THP1 cell lysate
Lane 2 : NCI-H460 lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa
Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)

Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution + K562 lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (ab186425)

Western blot analysis of NK-p30 in K562 cell lysate immunoprecipitated using ab186425 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.
Anti-NKp30 antibody [EPR14509] (ab186425)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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