Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NKp30 antibody [EPR14509] (ab186425)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14509] to NKp30
  • Suitable for: IP, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-NKp30 antibody [EPR14509]
    See all NKp30 primary antibodies

  • Description

    Rabbit monoclonal [EPR14509] to NKp30

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • THP1, NCI-H460, K562 and rat brain lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14509

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)
    Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)
    All lanes : Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution

    Lane 1 : THP1 cell lysate
    Lane 2 : NCI-H460 lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)
    Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)
    Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution + K562 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (ab186425)
    Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (ab186425)

    Western blot analysis of NK-p30 in K562 cell lysate immunoprecipitated using ab186425 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.

  • Anti-NKp30 antibody [EPR14509] (ab186425)
    Anti-NKp30 antibody [EPR14509] (ab186425)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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