Anti-NIT2 antibody (ab183074)
Key features and details
- Rabbit polyclonal to NIT2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NIT2 antibody
See all NIT2 primary antibodies -
Description
Rabbit polyclonal to NIT2 -
Host species
Rabbit -
Specificity
ab183074 detects endogenous level of total NIT2 protein -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide within Human NIT2 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Genbank: NP_064587
Database link: Q9NQR4 -
Positive control
- Mouse liver and kidney tissue lysates, mouse RAW264.7 cell lysate; Human colon cancer tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
(PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NIT2 antibody (ab183074)All lanes : Anti-NIT2 antibody (ab183074) at 1/950 dilution
Lane 1 : mouse liver tissue lysate
Lane 2 : mouse kidney tissue lysate
Lane 3 : RAW264.7 cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 90 seconds10%SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIT2 antibody (ab183074)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling NIT2 with ab183074 at a 1/40 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)
