Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
![Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)](https://www.abcam.com/ps/products/250/ab250274/Images/ab250274-405739-anti-nit1-antibody-epr14577-b-bsa-and-azide-free-western-blot.jpg "Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14577(B)] to NIT1 - BSA and Azide free
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free
See all NIT1 primary antibodies -
Description
Rabbit monoclonal [EPR14577(B)] to NIT1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250274 is the carrier-free version of ab180942. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250274 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14577(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)All lanes : Anti-NIT1 antibody [EPR14577(B)] (ab180942) at 1/10000 dilution
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?This data was developed using ab180942, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)](https://www.abcam.com/ps/products/250/ab250274/Images/ab250274-2-anti-nit1-antibody-epr14577-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)This data was developed using ab180942, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NIT1 with ab180942 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. -
![Immunoprecipitation - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)](https://www.abcam.com/ps/products/250/ab250274/Images/ab250274-1-anti-nit1-antibody-epr14577-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)This data was developed using ab180942, the same antibody clone in a different buffer formulation.Western blot analysis of fetal liver lysate immunoprecipitated using ab180942 at 1/60 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution. -
![Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)](https://www.abcam.com/ps/products/250/ab250274/Images/ab250274-4-benefits-of-recombinant-antibodies.png)
Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
