Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14577(B)] to NIT1 - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free
    See all NIT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14577(B)] to NIT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250274 is the carrier-free version of ab180942. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250274 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
    Western blot - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
    All lanes : Anti-NIT1 antibody [EPR14577(B)] (ab180942) at 1/10000 dilution

    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180942, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
    Flow Cytometry - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
    This data was developed using ab180942, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NIT1 with ab180942 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Immunoprecipitation - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
    Immunoprecipitation - Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
    This data was developed using ab180942, the same antibody clone in a different buffer formulation.Western blot analysis of fetal liver lysate immunoprecipitated using ab180942 at 1/60 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.
  • Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)
    Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free (ab250274)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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