Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [CL2237] to NIFK
Suitable for: IHC-P, ICC/IF, WB
Reacts with: Human
Isotype: IgG1

Product name

Anti-NIFK antibody [CL2237]
See all NIFK primary antibodies
Description

Mouse monoclonal [CL2237] to NIFK
Host species

Mouse
Tested applications

Suitable for: IHC-P, ICC/IF, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human NIFK aa 150 to the C-terminus.
Database link: Q9BYG3

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptidesequence IDYDFPSLILQKTES as determined by overlapping synthetic peptides.
Positive control
- IHC-P: Human skin, small intestine, pancreas, kidney, liver and fallopian tube tissues. WB: U-251 MG cell lysate. ICC/IF: U-2 OS cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL2237
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-NIFK antibody [CL2237] (ab244216)

PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for MK167 (Green) using ab244216 at 4 μg/ml in ICC/IF.

Microtublues are stained red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

Paraffin-embedded human fallopian tube tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

Paraffin-embedded human liver tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

Paraffin-embedded human kidney tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

Paraffin-embedded human pancreas tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

Paraffin-embedded human small intestine tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

Paraffin-embedded human skin tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Western blot - Anti-NIFK antibody [CL2237] (ab244216)

Anti-NIFK antibody [CL2237] (ab244216) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 34 kDa

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