Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NIFK antibody [CL2237] (ab244216)

Key features and details

  • Mouse monoclonal [CL2237] to NIFK
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-NIFK antibody [CL2237]
    See all NIFK primary antibodies

  • Description

    Mouse monoclonal [CL2237] to NIFK

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human NIFK aa 150 to the C-terminus.
    Database link: Q9BYG3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Binds to an epitope located within the peptidesequence IDYDFPSLILQKTES as determined by overlapping synthetic peptides.

  • Positive control

    • IHC-P: Human skin, small intestine, pancreas, kidney, liver and fallopian tube tissues. WB: U-251 MG cell lysate. ICC/IF: U-2 OS cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CL2237

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NIFK antibody [CL2237] (ab244216)
    Immunocytochemistry/ Immunofluorescence - Anti-NIFK antibody [CL2237] (ab244216)

    PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for MK167 (Green) using ab244216 at 4 μg/ml in ICC/IF.

    Microtublues are stained red.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

    Paraffin-embedded human fallopian tube tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

    Paraffin-embedded human liver tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

    Paraffin-embedded human kidney tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

    Paraffin-embedded human pancreas tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

    Paraffin-embedded human small intestine tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIFK antibody [CL2237] (ab244216)

    Paraffin-embedded human skin tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.

  • Western blot - Anti-NIFK antibody [CL2237] (ab244216)
    Western blot - Anti-NIFK antibody [CL2237] (ab244216)
    Anti-NIFK antibody [CL2237] (ab244216) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate

    Predicted band size: 34 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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