Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab110801)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody
See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies -
Description
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab168251) -
Positive control
- This antibody gave a positive signal in Human Spinal Cord tissue lysate.
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General notes
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1136 Human
- Omim: 118503 Human
- SwissProt: P32297 Human
- Unigene: 89605 Human
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Alternative names
- ACHA3_HUMAN antibody
- AChR antibody
- Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
see all
Images
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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab110801)Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 8 minutesThe band observed at 51 kDa could potentially be a cleaved form of v due to the presence of a 31 amino acid signal peptide. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab110801 has been referenced in 1 publication.
- Armstrong LC et al. High-Throughput Patch Clamp Screening in Human a6-Containing Nicotinic Acetylcholine Receptors. SLAS Discov 22:686-695 (2017). PubMed: 28298165
Images
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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab110801)Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 8 minutesThe band observed at 51 kDa could potentially be a cleaved form of v due to the presence of a 31 amino acid signal peptide. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
