Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

Key features and details

Mouse monoclonal [6D3A10] to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]
See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies
Description

Mouse monoclonal [6D3A10] to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 aa 32-240. (Expressed in E.coli).
Database link: CHRNA3
Positive control
- WB: Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein; Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. Flow: SH-SY5Y and SK-N-SH cells.
General notes

This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

6D3A10
Isotype

IgG1
Research areas

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution + Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein

Predicted band size: 57 kDa

Expected MW is 50.6 kDa.
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate.

Predicted band size: 57 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

SMMC-7721 (human hepatocarcinoma cell line) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution (green) compared with a negative control (red).
Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Nicotinic Acetylcholine Receptor alpha using ab233717 at 1/200 dilution (green) compared to a negative control (red).

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