Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443)
![Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443)](https://www.abcam.com/ps/products/151/ab151443/Images/ab151443-410172-anti-nherf-2sip-1-antibody-32b6-western-blot-human-knockout-lysate.jpg "Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443)")
Key features and details
- Mouse monoclonal [32B6] to NHERF-2/SIP-1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NHERF-2/SIP-1 antibody [32B6]
See all NHERF-2/SIP-1 primary antibodies -
Description
Mouse monoclonal [32B6] to NHERF-2/SIP-1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length protein corresponding to Human NHERF-2/SIP-1.
Sequence:MAAPEPLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAAL RAGDRLVEVNGVNVEGETHHQVVQRIKAVEGQTRLLVVDQETDEELRRRQ LTCTEEMAQRGLPPAHDPWEPKPDWAHTGSHSSEAGKKDVSGPLRELRPR LCHLRKGPQGYGFNLHSDKSRPGQYIRSVDPGSPAARSGLRAQDRLIEVN GQNVEGLRHAEVVASIKAREDEARLLVVDPETDEHFKRLRVTPTEEHVEG PLPSPVTNGTSPAQLNGGSACSSRSDLPGSDKDTEDGSAWKQDPFQESGL HLSPTAAEAKEKARAMRVNKRAPQMDWNRKREIFSNF
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Positive control
- WB: HEK293T, MCF7, T-47D and HaCat cell lysates.
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General notes
Previously labelled as SLC9A3R2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
32B6 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443)All lanes : Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SLC9A3R2 knockout HEK293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : T-47D cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab151443 observed at 45 kDa. Red - loading control ab52901 observed at kDa.
ab151443 Anti-NHERF-2/SIP-1 antibody [32B6] was shown to specifically react with NHERF-2/SIP-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266778 (knockout cell lysate ab258685) was used. Wild-type and NHERF-2/SIP-1 knockout samples were subjected to SDS-PAGE. ab151443 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443)](https://www.abcam.com/ps/products/151/ab151443/Images/SLC9A3R2-Primary-antibodies-ab151443-1.jpg)
Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443)Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443) at 1/100 dilution + HaCat Human keratinocyte cells
Predicted band size: 37 kDa
