Anti-Neuropeptide S antibody (ab18252)
Key features and details
- Rabbit polyclonal to Neuropeptide S
- Suitable for: IHC (PFA fixed), RIA
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Neuropeptide S antibody
See all Neuropeptide S primary antibodies -
Description
Rabbit polyclonal to Neuropeptide S -
Host species
Rabbit -
Specificity
This antibody is generated against the complete 20aa sequence of the fully processed rat NPS peptide, it should run at about 2kDa on WB. ab18252 detects mouse NPS peptide in IHC (PFA fixed) and in RIA in hypothalamic mouse brain tissue. -
Tested Applications & Species
See all applications and species dataApplication Species IHC (PFA fixed) Rat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of fully processed Rat Neuropeptide S.
Read Abcam's proprietary immunogen policy (Peptide available as ab18699.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC (PFA fixed) RatAll applications MouseApplication Abreviews Notes IHC (PFA fixed) Use a concentration of 0.3 µg/ml.RIA Use a concentration of 0.1 - 0.3 µg/ml.Notes IHC (PFA fixed)
Use a concentration of 0.3 µg/ml.RIA
Use a concentration of 0.1 - 0.3 µg/ml.Target
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Function
Modulates arousal and anxiety. May play an important anorexigenic role (By similarity). Binds to its receptor NPSR1 with nanomolar affinity to increase intracellular calcium concentrations (PubMed:15312648, PubMed:16790440). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100043254 Mouse
- Entrez Gene: 100360071 Rat
- SwissProt: P0C0P8 Mouse
- SwissProt: P0C0P7 Rat
- Unigene: 192092 Mouse
- Unigene: 234299 Rat
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Alternative names
- Neuropeptide S antibody
- Nps antibody
- NPS_HUMAN antibody
Images
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Immunohistochemistry (PFA fixed) - Anti-Neuropeptide S antibody (ab18252)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom.
Immunofluorescent staining using the Neuropeptide S antibody (ab18252) in the rat hypothalamus. Picture taken using objectives X20 (A) or X40 (B). The staining is observed in thin and varicose fibers. This staining is blocked by preincubation with immunising peptides (ab18698 and ab18699). ab18252 was used at 1/1000 (0.3µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
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Radioimmunoassay - Anti-Neuropeptide S antibody (ab18252)IC50: 46 fmoles; ab18252 used at a dilution of 1/3000; Tracer: [125I] Y10-hNPS, 12.000 cpm; Detection method: anti-rabbit SPA (Amersham).
Radioimmunoassay was performed in equilibrium, using the SPA technology (Amersham). 50µl antirabbit YSi SPA beads (RPN 140) were mixed with 50µl antiserum (1:3000), 50µl tracer (12,000 cpm) and with or without cold NPS peptide in increasing concentrations. All reagents were resuspended in 0.1M phosphate buffer, pH 7, 0.1% BSA. Incubation was for 48h at RT. Bound radioligand was detected by scintillation proximity in a beta counter (MicroBeta, Wallac).
Datasheets and documents
References (6)
ab18252 has been referenced in 6 publications.
- Tillmann S et al. Sustained overexpression of neuropeptide S in the amygdala reduces anxiety-like behavior in rats. Behav Brain Res 367:28-34 (2019). PubMed: 30914309
- Breton-Provencher V & Sur M Active control of arousal by a locus coeruleus GABAergic circuit. Nat Neurosci 22:218-228 (2019). IHC-FoFr ; Mouse . PubMed: 30643295
- Grund T et al. Neuropeptide S Activates Paraventricular Oxytocin Neurons to Induce Anxiolysis. J Neurosci 37:12214-12225 (2017). Rat . PubMed: 29118105
- Adori C et al. Neuropeptide S- and Neuropeptide S receptor-expressing neuron populations in the human pons. Front Neuroanat 9:126 (2015). IHC ; Rat . PubMed: 26441556
- Liu X et al. Molecular fingerprint of neuropeptide S-producing neurons in the mouse brain. J Comp Neurol 519:1847-66 (2011). Mouse . PubMed: 21452230
- Clark SD et al. Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry. J Comp Neurol 519:1867-93 (2011). Mouse . PubMed: 21452235
Images
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Immunohistochemistry (PFA fixed) - Anti-Neuropeptide S antibody (ab18252) This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom.
Immunofluorescent staining using the Neuropeptide S antibody (ab18252) in the rat hypothalamus. Picture taken using objectives X20 (A) or X40 (B). The staining is observed in thin and varicose fibers. This staining is blocked by preincubation with immunising peptides (ab18698 and ab18699). ab18252 was used at 1/1000 (0.3µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
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Radioimmunoassay - Anti-Neuropeptide S antibody (ab18252)
IC50: 46 fmoles; ab18252 used at a dilution of 1/3000; Tracer: [125I] Y10-hNPS, 12.000 cpm; Detection method: anti-rabbit SPA (Amersham).
Radioimmunoassay was performed in equilibrium, using the SPA technology (Amersham). 50µl antirabbit YSi SPA beads (RPN 140) were mixed with 50µl antiserum (1:3000), 50µl tracer (12,000 cpm) and with or without cold NPS peptide in increasing concentrations. All reagents were resuspended in 0.1M phosphate buffer, pH 7, 0.1% BSA. Incubation was for 48h at RT. Bound radioligand was detected by scintillation proximity in a beta counter (MicroBeta, Wallac).
