Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB2 antibody [EPR15569] (ab186748)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15569] to NDUFB2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDUFB2 antibody [EPR15569]
    See all NDUFB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15569] to NDUFB2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle, fetal heart, fetal brain, fetal kidney, HeLa and 293 lysates; Rat kidney, heart and brain lysates; Mouse heart, kidney and spleen lysates; Human endometrium tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/10000 dilution + Human fetal heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 12 kDa

  • Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    All lanes : Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (ab186748)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFB2 with ab186748 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Flow Cytometry - Anti-NDUFB2 antibody [EPR15569] (ab186748)

    Flow Cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling NDUFB2 with ab186748 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-NDUFB2 antibody [EPR15569] (ab186748)
    Anti-NDUFB2 antibody [EPR15569] (ab186748)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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