Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)
![Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)](https://www.abcam.com/ps/products/247/ab247510/Images/ab247510-410642-anti-ndufaf1-antibody-epr2796-bsa-and-azide-free-western-blot.jpg "Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2796] to NDUFAF1 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free
See all NDUFAF1 primary antibodies -
Description
Rabbit monoclonal [EPR2796] to NDUFAF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247510 is the carrier-free version of ab79826. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247510 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2796 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)All lanes : Anti-NDUFAF1 antibody [EPR2796] (ab79826) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?This data was developed using ab79826, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)](https://www.abcam.com/ps/products/247/ab247510/Images/ab247510-4-anti-ndufaf1-antibody-epr2796-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)This data was developed using ab79826, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling NDUFAF1 with purified 79826 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue). Control: PBS only -
![Flow Cytometry - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)](https://www.abcam.com/ps/products/247/ab247510/Images/ab247510-3-anti-ndufaf1-antibody-epr2796-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)This data was developed using ab79826, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NDUFAF1 (red) with ab79826 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)](https://www.abcam.com/ps/products/247/ab247510/Images/ab247510-2-anti-ndufaf1-antibody-epr2796-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510) -
![Immunocytochemistry - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)](https://www.abcam.com/ps/products/247/ab247510/Images/ab247510-1-anti-ndufaf1-antibody-epr2796-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510) -
![Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)](https://www.abcam.com/ps/products/247/ab247510/Images/ab247510-6-benefits-of-recombinant-antibodies.png)
Anti-NDUFAF1 antibody [EPR2796] - BSA and Azide free (ab247510)