Anti-NDP52 antibody (ab151256)
Key features and details
- Rabbit polyclonal to NDP52
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDP52 antibody
See all NDP52 primary antibodies -
Description
Rabbit polyclonal to NDP52 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human NDP52 aa 174-389.
Database link: Q13137 -
Positive control
- IHC-P: H441 xenograft. WB: Raji cells, Hu7 whole cell extract. ICC/IF: HeLa cells. IP: Jurkat cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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NDP52 was immunoprecipitated from Jurkat whole cell extracts with 5 μg ab151256. Western blot was performed on the immunoprecipitate using ab151256. EasyBlot anti-rabbit IgG was used as the secondary antibody.
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All lanes : Anti-NDP52 antibody (ab151256) at 1/2000 dilution
Lane 1 : Untreated Huh-7 whole cell extracts
Lane 2 : Huh-7 whole cell extracts treated with Thapsigargin for 12hrs
Lane 3 : Huh-7 whole cell extracts treated with Thapsigargin for 24hrs
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa10% SDS-PAGE.
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HeLa stained for NDP52 (green) using ab151256 at 1/1000 dilution in ICC/IF.
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Immunohistochemical analysis of paraffin embedded H441 xenograft labeling NDP52 with ab151256 antibody at 1/100.
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Anti-NDP52 antibody (ab151256) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE