Anti-NCX1 antibody [EPR12739] (ab177952)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12739] to NCX1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NCX1 antibody [EPR12739]
See all NCX1 primary antibodies -
Description
Rabbit monoclonal [EPR12739] to NCX1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide within Human NCX1 aa 50-150. The exact sequence is proprietary.
Database link: P32418 -
Positive control
- Human, Rat and Mouse heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12739 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NCX1 antibody [EPR12739] (ab177952) (at a dilution of 1/1000 or 1/10000. Purified)
Lane 1 : Mouse heart lysates
Lane 2 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This antibody detects high backgroud at high working concentration.
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Anti-NCX1 antibody [EPR12739] (ab177952) (at a dilution of 1/1000 or 1/10000. Purified) + Human heart lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This antibody detects high backgroud at high working concentration.
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