Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)
![Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)](https://www.abcam.com/ps/products/250/ab250099/Images/ab250099-405585-anti-ncf1-p47-phox-antibody-epr13134-bsa-and-azide-free-western-blot.jpg "Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13134] to NCF1/p47-phox - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free
See all NCF1/p47-phox primary antibodies -
Description
Rabbit monoclonal [EPR13134] to NCF1/p47-phox - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment within Human NCF1/p47-phox. The exact sequence is proprietary.
Database link: P14598 -
General notes
ab250099 is the carrier-free version of ab179457 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250099 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13134 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)All lanes : Anti-NCF1/p47-phox antibody [EPR13134] (ab179457) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDaThis data was developed using ab179457, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)](https://www.abcam.com/ps/products/250/ab250099/Images/ab250099-399602-anti-ncf1-p47-phox-antibody-epr13134-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)This data was developed using ab179457, the same antibody clone in a different buffer formulation.
Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1/p47-phox, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)](https://www.abcam.com/ps/products/250/ab250099/Images/ab250099-3-benefits-of-recombinant-antibodies.png)
Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)
