Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13134] to NCF1/p47-phox - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free
    See all NCF1/p47-phox primary antibodies

  • Description

    Rabbit monoclonal [EPR13134] to NCF1/p47-phox - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human NCF1/p47-phox. The exact sequence is proprietary.
    Database link: P14598

  • General notes

    ab250099 is the carrier-free version of ab179457 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250099 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)
    Western blot - Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)
    All lanes : Anti-NCF1/p47-phox antibody [EPR13134] (ab179457) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa



    This data was developed using ab179457, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)
    Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)

    This data was developed using ab179457, the same antibody clone in a different buffer formulation.

    Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1/p47-phox, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)
    Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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