Anti-NAP1L4 antibody (ab21631)
Key features and details
- Rabbit polyclonal to NAP1L4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAP1L4 antibody -
Description
Rabbit polyclonal to NAP1L4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAP1L4.
Read Abcam's proprietary immunogen policy (Peptide available as ab22419.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications DogApplication Abreviews Notes WB (1) 1/250 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 43 kDa).Notes WB
1/250 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 43 kDa).Target
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Relevance
This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4676 Human
- Omim: 601651 Human
- SwissProt: Q99733 Human
- Unigene: 501684 Human
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Alternative names
- NAP2 antibody
- Nucleosome assembly protein 1-like 4 antibody
- Nucleosome assembly protein 2 antibody
Images
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Western blot - Anti-NAP1L4 antibody (ab21631)Lanes 1-2 : Anti-NAP1L4 antibody (ab21631) at 1/1000 dilution
Lanes 3-4 : Anti-NAP1L4 antibody (ab21631) at 1/500 dilution
Lanes 5-6 : Anti-NAP1L4 antibody (ab21631) at 1/250 dilution
Lanes 1 & 3 & 5 : U2OS whole cell lysate
Lanes 2 & 4 & 6 : U2OS whole cell lysate, treated with ionizing radiation
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity), 64 kDa (possible cross reactivity)For each lane, U2OS cells, either treated or not treated with ionizing radiation, were scraped from a 60 mm dish and added to 75 µl of 2X Laemmli buffer. 20 µl of these samples were loaded into each lane. ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.
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Western blot - Anti-NAP1L4 antibody (ab21631)All lanes : Anti-NAP1L4 antibody (ab21631) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity)
ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.
Datasheets and documents
References (3)
ab21631 has been referenced in 3 publications.
- Spiniello M et al. HyPR-MS for Multiplexed Discovery of MALAT1, NEAT1, and NORAD lncRNA Protein Interactomes. J Proteome Res 17:3022-3038 (2018). PubMed: 29972301
- Aslanian A et al. Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells. DNA Repair (Amst) 15:29-38 (2014). PubMed: 24461736
- Cho I et al. ATP-dependent chromatin remodeling by Cockayne syndrome protein B and NAP1-like histone chaperones is required for efficient transcription-coupled DNA repair. PLoS Genet 9:e1003407 (2013). IP ; Human . PubMed: 23637612
Images
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Western blot - Anti-NAP1L4 antibody (ab21631)Lanes 1-2 : Anti-NAP1L4 antibody (ab21631) at 1/1000 dilution
Lanes 3-4 : Anti-NAP1L4 antibody (ab21631) at 1/500 dilution
Lanes 5-6 : Anti-NAP1L4 antibody (ab21631) at 1/250 dilution
Lanes 1 & 3 & 5 : U2OS whole cell lysate
Lanes 2 & 4 & 6 : U2OS whole cell lysate, treated with ionizing radiation
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity), 64 kDa (possible cross reactivity)For each lane, U2OS cells, either treated or not treated with ionizing radiation, were scraped from a 60 mm dish and added to 75 µl of 2X Laemmli buffer. 20 µl of these samples were loaded into each lane. ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.
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Western blot - Anti-NAP1L4 antibody (ab21631)All lanes : Anti-NAP1L4 antibody (ab21631) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity)
ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.