Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)
![Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)](https://www.abcam.com/ps/products/251/ab251343/Images/ab251343-403372-anti-nabc1-antibody-epr15441-2-bsa-and-azide-free-western-blot.jpg "Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15441(2)] to NABC1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free
See all NABC1 primary antibodies -
Description
Rabbit monoclonal [EPR15441(2)] to NABC1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251343 is the carrier-free version of ab201455. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251343 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15441(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)All lanes : Anti-NABC1 antibody [EPR15441(2)] (ab201455) at 1/5000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 2 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 4 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 120,62 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab201455, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID: 14567997. Please see page 6. The band observed at 120 kDa may represent a homodimer of NABC1, this information is based on SwissProt (Section: Subunit Structure).
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![Western blot - Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)](https://www.abcam.com/ps/products/251/ab251343/Images/ab251343-399283-anti-nabc1-antibody-epr15441-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)Anti-NABC1 antibody [EPR15441(2)] (ab201455) at 1/1000 dilution + Human testis lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 120,62 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab201455, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID: 14567997. Please see page 6. The band observed at 120 kDa may represent a homodimer of NABC1, this information is based on SwissProt (Section: Subunit Structure).
-
![Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)](https://www.abcam.com/ps/products/251/ab251343/Images/ab251343-3-benefits-of-recombinant-antibodies.png)
Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (ab251343)
