Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)
![Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)](https://www.abcam.com/ps/products/208/ab208577/Images/ab208577-255281-anti-n6-methyladenosine-m6a-antibody-17-3-4-1-immunoprecipitation.jpg "Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)")
Key features and details
- Mouse monoclonal [17-3-4-1] to N6-methyladenosine (m6A)
- Suitable for: Northwestern, IP, Southern Blot
- Reacts with: Mouse, Human, Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-N6-methyladenosine (m6A) antibody [17-3-4-1]
See all N6-methyladenosine (m6A) primary antibodies -
Description
Mouse monoclonal [17-3-4-1] to N6-methyladenosine (m6A) -
Host species
Mouse -
Specificity
This antibody recognises N6-methyladenosine in both modified RNA and DNA. -
Tested applications
Suitable for: Northwestern, IP, Southern Blotmore details -
Species reactivity
Reacts with: Mouse, Human, Saccharomyces cerevisiae -
Immunogen
Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to bovine serum albumin. Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine
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Positive control
- methylated RNA (negative control non methylated RNA); single stranded DNA;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
17-3-4-1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)
N6-methyladenosine (m6A) was immunoprecipitated using ab208577 from radiolabeled synthetic methylated transcript (Bound); radiolabeled Non methylated control (Non bound) and radiolabeled Input RNA control.
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![Northwestern blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)](https://www.abcam.com/ps/products/208/ab208577/Images/ab208577-255280-anti-n6-methyladenosine-m6a-antibody-17-3-4-1-northwestern-blot.jpg)
Northwestern blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)Use of ab208577 for northwestern blot using synthetic methlyated and non methlyated RNA.
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![Southern Blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)](https://www.abcam.com/ps/products/208/ab208577/Images/ab208577-256037-anti-n6-methyladenosine-m6a-antibody-17-3-4-1-southern-blot.jpg)
Southern Blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)Lane 1 Single stranded DNA
Lane 2 Double stranded DNA
Labelled with ab208577. Arrow shows m6A containing fragment.
