Anti-Myosin IXA antibody (ab241200)
Key features and details
- Rabbit polyclonal to Myosin IXA
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Myosin IXA antibody
See all Myosin IXA primary antibodies -
Description
Rabbit polyclonal to Myosin IXA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Myosin IXA aa 2350-2400. The exact sequence is proprietary.
Database link: B2RTY4 -
Positive control
- IP: HeLa cell lysate.
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General notes
ab241200 has not performed satisfactorily when used for WB of Myosin IXA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Myosin IXA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Myosin IXA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Myosin IXA antibody (ab241200)
Myosin IXA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241200 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241200 at 1/1000 dilution.
Lane 1: ab241200 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
