Anti-Myelin Basic Protein antibody [MBP2] (ab11159) at 1/1000 dilution + human brain tissue lysate at 20 µg

Secondary
Anti-mouse IgG for IP (HRP) (ab131368) at 1/50000 dilution

Predicted band size: 33 kDa


Exposure time: 26 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/1000 (1.125 ug/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the human cerebrum. The section was incubated with ab11159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10 (112.5 ug/ml) dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Positive staining on the rat cerebrum. The section was incubated with ab11159 for 30 mins at room temperature and blocked mouse IgG by specific antibody ab125913 for 8mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Positive staining on the mouse cerebrum. The section was incubated with ab11159 for 30 mins at room temperature and blocked mouse IgG by specific antibody ab125913 for 8mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myelin Basic Protein with ab11159 at 1:1000 (1.125 μg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control: No staining on human spleen (PMID: 9518636). The section was incubated with ab11159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.