Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23594-106] to MVP - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MVP antibody [EPR23594-106] - BSA and Azide free
    See all MVP primary antibodies

  • Description

    Rabbit monoclonal [EPR23594-106] to MVP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, A431, Caco-2, HeLa treated with 100U/ml IFN gamma for 24 hours, whole, RAW 264.7, NIH/3T3, C6 and PC-12 whole cell lysate. Human colon, lung and kidney tissue lysate. Mouse colon and kidney tissue lysate. IHC-P: Human cerebrum tissue. Human lung cancer and ovary cancer tissue. ICC/IF: A549 and NIH/3T3 cells. Flow Cyt: A549 cells.

  • General notes

    ab273097 is the carrier-free version of ab273093. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Western blot - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    All lanes : Anti-MVP antibody [EPR23594-106] (ab273093) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
    Lane 2 : MVP knockout HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
    Lane 3 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 99 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab273093).

    Lanes 1-3: Merged signal (red and green). Green - ab273093 observed at 110 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    ab273093 Anti-MVP antibody [EPR23594-106] was shown to specifically react with MVP in wild-type HeLa cells in Western blot. Significant decrease (8.7 % of intensity compared to the WT band) of signal was observed when MVP knockout cell line ab264817 (knockout cell lysate ab257544) was used.

    ab273093 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling MVP with ab273093 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human ovary cancer (PMID: 23739867). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273093).

  • Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 cells labelling MVP with ab273093 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in A549 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273093).

  • Flow Cytometry - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Flow Cytometry - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling MVP with ab273093 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273093).

  • Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling MVP with ab273093 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273093).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling MVP with ab273093 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human lung cancer (PMID: 22117969). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273093).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MVP with ab273093 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in endothelium of human cerebrum (PMID: 14636345). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273093).

  • Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
    Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)

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