Anti-MUSK antibody (ab92950)
Key features and details
- Rabbit polyclonal to MUSK
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MUSK antibody
See all MUSK primary antibodies -
Description
Rabbit polyclonal to MUSK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human MUSK aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102603) -
Positive control
- This antibody gave a positive signal in Human and Mouse Skeletal Muscle Tissue Lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).Target
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Function
Receptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization. -
Involvement in disease
Defects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 FZ (frizzled) domain.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4593 Human
- Entrez Gene: 18198 Mouse
- Omim: 601296 Human
- SwissProt: O15146 Human
- SwissProt: Q61006 Mouse
- Unigene: 521653 Human
- Unigene: 16148 Mouse
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Alternative names
- CMS9 antibody
- FADS antibody
- MDK 4 antibody
see all
Images
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Western blot - Anti-MUSK antibody (ab92950)All lanes : Anti-MUSK antibody (ab92950) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
MUSK protein sequence contains two potential glycosylation sites (SwissProt Data), which may explain the migration of the band at a higher molecular weight than predicted.
Datasheets and documents
References (2)
ab92950 has been referenced in 2 publications.
- Kelly NA et al. Effects of aging and Parkinson's disease on motor unit remodeling: influence of resistance exercise training. J Appl Physiol (1985) 124:888-898 (2018). PubMed: 29357501
- Logan CV et al. Congenital Myasthenic Syndrome Type 19 Is Caused by Mutations in COL13A1, Encoding the Atypical Non-fibrillar Collagen Type XIII a1 Chain. Am J Hum Genet 97:878-85 (2015). PubMed: 26626625
Images
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Western blot - Anti-MUSK antibody (ab92950)All lanes : Anti-MUSK antibody (ab92950) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
MUSK protein sequence contains two potential glycosylation sites (SwissProt Data), which may explain the migration of the band at a higher molecular weight than predicted.
