Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP184E] to muscle Actin - BSA and Azide free
  • Suitable for: IHC-P, IHC-FoFr, Flow Cyt, WB, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-muscle Actin antibody [EP184E] - BSA and Azide free
    See all muscle Actin primary antibodies

  • Description

    Rabbit monoclonal [EP184E] to muscle Actin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This Actin antibody recognizes all muscle actin.

  • Tested applications

    Suitable for: IHC-P, IHC-FoFr, Flow Cyt, WB, ICCmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247305 is the carrier-free version of ab46805. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247305 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Western blot - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Anti-muscle Actin antibody [EP184E] (ab46805) at 1/1000 dilution + A431 cell lysate at 10ug/lane

    Secondary
    Goat anti-rabbit, HRP labelled. at 1/2000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    This data was developed using ab46805, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Immunocytochemistry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab46805 at a 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin ab46805 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Flow Cytometry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab46805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab46805, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using ab46805. Green-specific fluorescent staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)

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