Antibodies, Proteins, Kits and Reagents for Life Science

381 945 ₸ Product size

100 µg 381 945 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [5B12] to MUC4
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human
Isotype: IgG2a

Product name

Anti-MUC4 antibody [5B12]
See all MUC4 primary antibodies
Description

Mouse monoclonal [5B12] to MUC4
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment with tag: GVSLFPYGAD AGDLEFVRRT VDFTSPLFKP ATGFPLGSSL RDSLYFTDNG QIIFPESDYQ IFSYPNPLPT GFTGRDPVAL VAPFWDDADF STGRGTTFYQ EYETFYGEHS , corresponding to amino acids 79-189 of Human MUC4

Run BLAST with Run BLAST with
Positive control
- WB: HeLa cell lysate. IHC-P: Human stomach tissue. Flow Cyt: A549 cells.
General notes

This product was changed from ascites to tissue culture supernatant on 12 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.4
Constituent: PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

5B12
Isotype

IgG2a
Research areas

Western blot - Anti-MUC4 antibody [5B12] (ab60720)

Anti-MUC4 antibody [5B12] (ab60720) at 1 µg/ml + HeLa cell lysate at 25 µg

Predicted band size: 235 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [5B12] (ab60720)

MUC4 antibody (ab60720) used in immunohistochemistry at 3ug/mL on formalin fixed and paraffin embedded human stomach.

This image was generated using the ascites version of the product.
Flow Cytometry - Anti-MUC4 antibody [5B12] (ab60720)

Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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