Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
Key features and details
- Goat polyclonal to alpha Defensin 1 + 2 + 3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Defensin 1 + 2 + 3 antibody -
Description
Goat polyclonal to alpha Defensin 1 + 2 + 3 -
Host species
Goat -
Specificity
ab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
C-DESLAPKHPGSRKN
, corresponding to internal sequence amino acids 50-63 of Human alpha Defensin 1 + 2 + 3 (NP_004075.1; NP_005208.1). -
Positive control
- Human spleen lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)Immunohistochemical analysis of paraffin embedded Human Thymus staining alpha Defensin 1 + 2 + 3 with ab99504 (3.8µg/ml). Steamed antigen retrieval with citrate buffer pH 6.
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Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 10 kDa
Exposure time: 1 hour
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)ab99504 (3.8µg/ml) staining of paraffin embedded Human Thymus shows staining of selected cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
