Anti-MTTP/MTP antibody (ab63467)
Key features and details
- Rabbit polyclonal to MTTP/MTP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTTP/MTP antibody
See all MTTP/MTP primary antibodies -
Description
Rabbit polyclonal to MTTP/MTP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human Liver Tissue Lysate.
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General notes
This product was previously labelled as MTTP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications CowApplication Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).Target
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Function
Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. -
Tissue specificity
Liver and small intestine. Also found in ovary, testis and kidney. -
Involvement in disease
Abetalipoproteinemia -
Sequence similarities
Contains 1 vitellogenin domain. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 4547 Human
- Omim: 590075 Human
- SwissProt: P55156 Cow
- SwissProt: P55157 Human
- Unigene: 195799 Human
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Alternative names
- ABL antibody
- Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
- Microsomal triglyceride transfer protein 88kD antibody
see all
Images
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Western blot - Anti-MTTP/MTP antibody (ab63467)Anti-MTTP/MTP antibody (ab63467) at 1 µg/ml + Liver (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-MTTP/MTP antibody (ab63467)ICC/IF image of ab63467 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63467 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5min) HeLa cell types at 1ug/ml
Protocols
Datasheets and documents
References (3)
ab63467 has been referenced in 3 publications.
- Hu L et al. Avasimibe: A novel hepatitis C virus inhibitor that targets the assembly of infectious viral particles. Antiviral Res 148:5-14 (2017). PubMed: 29074218
- Bai J et al. Sodium sulfite promotes the assembly and secretion of very low-density lipoprotein in HL-7702 hepatocytes. Toxicol Rep 3:98-104 (2016). PubMed: 28959527
- Yamamoto Y et al. Rapid alternative absorption of dietary long-chain fatty acids with upregulation of intestinal glycosylated CD36 in liver cirrhosis. Am J Clin Nutr 96:90-101 (2012). Human . PubMed: 22648712
Images
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Western blot - Anti-MTTP/MTP antibody (ab63467)Anti-MTTP/MTP antibody (ab63467) at 1 µg/ml + Liver (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 20 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-MTTP/MTP antibody (ab63467)
ICC/IF image of ab63467 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63467 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5min) HeLa cell types at 1ug/ml
