Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19781] to MTHFR - BSA and Azide free
  • Suitable for: WB, IP, ICC, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MTHFR antibody [EPR19781] - BSA and Azide free
    See all MTHFR primary antibodies

  • Description

    Rabbit monoclonal [EPR19781] to MTHFR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain, fetal heart, fetal kidney, spleen and fetal liver lysates; Jurkat, HT-29, HEK-293, RAW 264.7 and NIH/3T3 whole cell lysates; Mouse placenta and kidney lysates. ICC/IF: Jurkat and RAW 264.7 cells. IP: HT-29 whole cell lysate. IHC-P: Human thyroid carcinoma and kidney tissues.

  • General notes

    Ab251399 is the carrier-free version of ab203786. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Western blot - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    All lanes : Anti-MTHFR antibody [EPR19781] (ab203786) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa



    This data was developed using ab203786, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-4: 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)

    This data was developed using ab203786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MTHFR with ab203786 at 1/1000 (0.509 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human kidney. The section was incubated with ab203786 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)

    This data was developed using ab203786, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling MTHFR with ab203786 at 1/1000 (0.509 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human thyroid carcinoma. The section was incubated with ab203786 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

  • Western blot - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Western blot - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Lanes 1-3 : Anti-MTHFR antibody [EPR19781] (ab203786) at 1/1000 dilution
    Lanes 4-5 : Anti-MTHFR antibody [EPR19781] (ab203786) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : Human fetal liver tissue lysate
    Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 5 : Mouse placenta tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa


    Exposure time: 3 minutes


    This data was developed using ab203786, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Western blot - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    All lanes : Anti-MTHFR antibody [EPR19781] (ab203786) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa


    Exposure time: 3 minutes


    This data was developed using ab203786, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Immunocytochemistry - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    This data was developed using ab203786, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling MTHFR with ab203786 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab203786 at 1/100 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunocytochemistry - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Immunocytochemistry - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    This data was developed using ab203786, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling MTHFR with ab203786 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab203786 at 1/100 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunoprecipitation - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Immunoprecipitation - Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    This data was developed using ab203786, the same antibody clone in a different buffer formulation.MTHFR was immunoprecipitated from 0.35mg of HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate with ab203786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203786 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: HT-29 whole cell lysate, 10µg (Input). Lane 2: ab203786 IP in HT-29 whole cell lysate. Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab203786 in HT-29 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)
    Anti-MTHFR antibody [EPR19781] - BSA and Azide free (ab251399)

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