Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)
![Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)](https://www.abcam.com/ps/products/76/ab76579/Images/ab76579-92080-anti-mst4-mst3-stk25-phospho-t174-t178-t190-antibody-ep2123y-western-blot.gif "Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2123Y] to MST4 + MST3 + STK25 (phospho T174 + T178 + T190)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y]
See all MST4 + MST3 + STK25 primary antibodies -
Description
Rabbit monoclonal [EP2123Y] to MST4 + MST3 + STK25 (phospho T174 + T178 + T190) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A phospho specific peptide corresponding to residues surrounding threonine 178 of human MST4, theonine 190 of human MST3 and threonine 174 of human STK25.
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Positive control
- HeLa cell lysates treated with calyculin A.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2123Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)All lanes : Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579) at 1/2000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 47-49 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
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![Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)](https://www.abcam.com/ps/products/76/ab76579/Images/ab76579-2-benefits-of-recombinant-antibodies.png)
Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)