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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

Price and availability

606 422 ₸

Availability

Order now and get it on Thursday June 01, 2023

Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E375] to MSK1 (phospho S376) - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free
    See all MSK1 primary antibodies
  • Description

    Rabbit monoclonal [E375] to MSK1 (phospho S376) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma. ICC/IF: HepG2 cells
  • General notes

    ab210529 is the carrier-free version of ab32190.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E375
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Images

  • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

    All lanes : HEK-293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 90 kDa


    Exposure time: 10 seconds


    This data was developed using ab32190, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed bands: 90, 80 kDa.

    The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

  • Immunocytochemistry - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    Immunocytochemistry - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

    Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.

    Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37℃ 1h).

    This data was developed using ab32190, the same antibody clone in a different buffer formulation.

     

  • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

    Lane 1 : Untreated HEK293 (human embryonic kidney) whole cell lysate
    Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate
    Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 90 kDa


    Exposure time: 5 seconds


    This data was developed using ab32190, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed band: 90, 80 kDa.

    The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

  • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution

    Lane 1 : Untreated K562 cell lysate
    Lane 2 : K562 cell lysate treated with Alkaline

    Predicted band size: 90 kDa
    Observed band size: 87 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32190, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    This data was developed using ab32190, the same antibody clone in a different buffer formulation.Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
    Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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