Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20920-19] to MPTX+MPTX2 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free
    See all MPTX+MPTX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20920-19] to MPTX+MPTX2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human MPTX recombinant protein. His-lif tagged human MPTX2 recombinant protein. Wild type mouse large intestine tissue lysate. IHC-Fr: Mouse small intestine tissue. IHC-P: Mouse small intestine tissue. ICC/IF: HEK-293T cells transfected with a flag-tagged MPTX expression construct. Flow Cyt: HEK-293T cells transfected with a flag-tagged MPTX expression construct.

  • General notes

    ab269857 is the carrier-free version of ab238123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20920-19

  • Isotype

    IgG

Images

  • Immunohistochemistry (Frozen sections) - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)
    Immunohistochemistry (Frozen sections) - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labeling MPTX1 with ab238123 at 1/500 (1.10 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    Positive staining on Paneth cells in mouse small intestine is observed (PMID: 29144463).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238123).

  • Flow Cytometry - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)
    Flow Cytometry - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with a flag-tagged MPTX expression construct cells labeling MPTX1 with ab238123 at 1/500 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). A Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238123).

  • Immunocytochemistry/ Immunofluorescence - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)
    Immunocytochemistry/ Immunofluorescence - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with a flag-tagged MPTX expression construct cells labeling MPTX1 with ab238123 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with a flag-tagged MPTX expression construct.

    Mouse monoclonal anti-DDDDK antibody followed by ab150120 AlexaFluor®594 Goat anti-Mouse secondary was used to counterstain at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    -ve control 1: ab238123 at a 1/50 dilution followed by ab150120 at a 1/1000 dilution.

    -ve control 2: Mouse monoclonal anti-DDDDK antibody at a 1/500 dilution followed by ab150077 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238123).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling MPTX1 with ab238123 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on Paneth cells of mouse small intestine is observed. The section was incubated with ab238123 for 5 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238123).

  • Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)
    Anti-MPTX+MPTX2 antibody [EPR20920-19] - BSA and Azide free (ab269857)

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