Anti-mono methyl Arginine antibody [5D1] (ab415)
![Anti-mono methyl Arginine antibody [5D1] (ab415)](https://www.abcam.com/ps/products/0/ab415/Images/ab415_1.jpg "Anti-mono methyl Arginine antibody [5D1] (ab415)")
Key features and details
- Mouse monoclonal [5D1] to mono methyl Arginine
- Suitable for: WB, ChIP
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-mono methyl Arginine antibody [5D1]
See all mono methyl Arginine primary antibodies -
Description
Mouse monoclonal [5D1] to mono methyl Arginine -
Host species
Mouse -
Tested applications
Suitable for: WB, ChIPmore details -
Species reactivity
Reacts with: Species independent -
General notes
This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status. -
Clonality
Monoclonal -
Clone number
5D1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415) This image is courtesy of Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UK
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.
Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5
µ l of ab415 and 4x106 cells were used in each ChIP experiment. -
![ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)](https://www.abcam.com/ps/products/0/ab415/Images/ab415-3508-anti-mono-methyl-arginine-antibody-5d1-chip.jpg)
ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.
