Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLKL antibody [3H1] - BSA and Azide free (ab255747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [3H1] to MLKL - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MLKL antibody [3H1] - BSA and Azide free
    See all MLKL primary antibodies

  • Description

    Rat monoclonal [3H1] to MLKL - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Mouse MLKL aa 100-200. KLH-conjugated linear peptide corresponding to a sequence from the two-helix linker (Brace) region N-terminal to the pseudokinase domain of mouse MLKL.
    Database link: Q9D2Y4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HUVEC, HT-29, HeLa, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255747 is the carrier-free version of ab243142. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MLKL antibody [3H1] - BSA and Azide free (ab255747)
    Western blot - Anti-MLKL antibody [3H1] - BSA and Azide free (ab255747)
    All lanes : Anti-MLKL antibody [3H1] (ab243142) at 1/2000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 7 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution

    Predicted band size: 54 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 5 secs; Lanes 4/5/7: 3 secs; Lane 6: 15 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab243142).

  • Anti-MLKL antibody [3H1] - BSA and Azide free (ab255747)
    Anti-MLKL antibody [3H1] - BSA and Azide free (ab255747)

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