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Anti-MKLP1 antibody [EPR10879] (ab174304)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-MKLP1 antibody [EPR10879] (ab174304)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10879] to MKLP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-MKLP1 antibody [EPR10879]
    See all MKLP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10879] to MKLP1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human MKLP1 aa 800-900 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q02241

  • Positive control

    • HeLa, 293T and A549 cell lysates. A547 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10879
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin-like
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Myocardial regeneration

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] (ab174304)

    Immunofluorescence staining of HeLa cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified) + RAW264.7 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 110 kDa
    Observed band size: 98 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (purified) + Rat lung tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 110 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/20000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 110 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified)

    Lane 1 : HEK293 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)
    All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 110 kDa
    Additional bands at: 98-110 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] (ab174304)
    Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] (ab174304)

    Immunofluorescent analysis of A547 cells labeling MKLP1 with unpurified ab174304 at 1/100.

  • Anti-MKLP1 antibody [EPR10879] (ab174304)
    Anti-MKLP1 antibody [EPR10879] (ab174304)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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