Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5357] to MK2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MK2 antibody [EPR5357] - BSA and Azide free
    See all MK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5357] to MK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248443 is the carrier-free version of ab133279 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248443 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as MAPKAP Kinase 2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)
    Western blot - Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)

    This data was developed using ab133279, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133279 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133279 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab133279 and ab8245 (loading control to GAPDH) were diluted to 1/000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)
    Western blot - Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)
    All lanes : Anti-MK2 antibody [EPR5357] (ab133279) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa



    This data was developed using ab133279, the same antibody clone in a different buffer formulation.

     

  • Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)
    Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)

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