Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)
![Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)](https://www.abcam.com/ps/products/248/ab248443/Images/ab248443-403344-anti-mk2-antibody-epr5357-bsa-and-azide-free-western-blot.jpg "Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5357] to MK2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MK2 antibody [EPR5357] - BSA and Azide free
See all MK2 primary antibodies -
Description
Rabbit monoclonal [EPR5357] to MK2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248443 is the carrier-free version of ab133279 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248443 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as MAPKAP Kinase 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5357 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)
This data was developed using ab133279, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133279 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133279 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab133279 and ab8245 (loading control to GAPDH) were diluted to 1/000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)](https://www.abcam.com/ps/products/248/ab248443/Images/ab248443-399175-anti-mk2-antibody-epr5357-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)All lanes : Anti-MK2 antibody [EPR5357] (ab133279) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDaThis data was developed using ab133279, the same antibody clone in a different buffer formulation.
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![Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)](https://www.abcam.com/ps/products/248/ab248443/Images/ab248443-3-benefits-of-recombinant-antibodies.png)
Anti-MK2 antibody [EPR5357] - BSA and Azide free (ab248443)
