Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MIOX antibody [EPR17173] - BSA and Azide free (ab251262)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17173] to MIOX - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-MIOX antibody [EPR17173] - BSA and Azide free
    See all MIOX primary antibodies

  • Description

    Rabbit monoclonal [EPR17173] to MIOX - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251262 is the carrier-free version of ab198997. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251262 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MIOX antibody [EPR17173] - BSA and Azide free (ab251262)
    Western blot - Anti-MIOX antibody [EPR17173] - BSA and Azide free (ab251262)
    All lanes : Anti-MIOX antibody [EPR17173] (ab198997) at 1/2000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 10 seconds


    This data was developed using ab198997, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    MIOX is specifically expressed in the kidney. Lane 2, Human fetal heart, represents a negative control.

  • Immunoprecipitation - Anti-MIOX antibody [EPR17173] - BSA and Azide free (ab251262)
    Immunoprecipitation - Anti-MIOX antibody [EPR17173] - BSA and Azide free (ab251262)

    This data was developed using ab198997, the same antibody clone in a different buffer formulation.

    MIOX was immunoprecipitated from 1mg of Human fetal kidney whole cell extract with ab198997 at 1/150 dilution. Western blot was performed using ab198997 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal kidney whole cell extract 10µg (Input).

    Lane 2: ab198997 IP in Human fetal kidney whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198997 in Human fetal kidney whole cell extract.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-MIOX antibody [EPR17173] - BSA and Azide free (ab251262)
    Anti-MIOX antibody [EPR17173] - BSA and Azide free (ab251262)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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