Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP15279] to Mimitin - C-terminal
  • Suitable for: ICC/IF, IP, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Mimitin antibody [EP15279] - C-terminal
    See all Mimitin primary antibodies

  • Description

    Rabbit monoclonal [EP15279] to Mimitin - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 cells and cell lysates: HeLa, SH-SY5Y and HEL cell lysates: Human fetal heart, Mouse kidney and Mouse heart lysates: Human liver and Mouse liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : HEL cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution + Human fetal heart lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Mimitin with ab192267 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue). 
    Negative controls, ab192267 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

  • Immunoprecipitation - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunoprecipitation - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Western blot analysis of Mimitin in immunoprecipitation pellets from MDA-MB-435 lysate. ab192267 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

  • Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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