Anti-MHC class I antibody [R1-9.6] - Chimeric (ab281901)
![Anti-MHC class I antibody [R1-9.6] - Chimeric (ab281901)](https://www.abcam.com/ps/products/281/ab281901/Images/ab281901-1-AntiMHC-class-I-antibody-immunocytochemistry-splenocytes-mouse.jpg "Anti-MHC class I antibody [R1-9.6] - Chimeric (ab281901)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [R1-9.6] to MHC class I - Chimeric
- Suitable for: ICC
- Reacts with: Mouse
Overview
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Product name
Anti-MHC class I antibody [R1-9.6] - Chimeric
See all MHC class I primary antibodies -
Description
Rabbit monoclonal [R1-9.6] to MHC class I - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC class I. Raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
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Positive control
- ICC: Mouse Splenocytes.
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General notes
ab281901 is the chimeric rabbit version of ab281903.
This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
R1-9.6 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-MHC class I antibody [R1-9.6] (ab281901)
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes labelling MHC-I with ab281901 at 10 µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/ top-left). Image showing membrane staining in a subset of cells. The nuclear counter stain is DAPI (blue). The isotype control was anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody. Mouse splenocytes were immobilized on cover-slips.
