Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-METTL14 antibody [CL4254] (ab220031)

Key features and details

  • Mouse monoclonal [CL4254] to METTL14
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-METTL14 antibody [CL4254]
    See all METTL14 primary antibodies

  • Description

    Mouse monoclonal [CL4254] to METTL14

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment (proprietary-tag) corresponding to Human METTL14 aa 1-100.
    Database link: Q9HCE5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human endometrium, Human testis, Human fallopian tubes, Human prostate and Human duodenum tissues; A431 cell line lysate. ICC/IF: RH-30 cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CL4254

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-METTL14 antibody [CL4254] (ab220031)
    Western blot - Anti-METTL14 antibody [CL4254] (ab220031)
    Anti-METTL14 antibody [CL4254] (ab220031) at 1/500 dilution + A431 cell line lysate

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human duodenum tissue labeling METTL14 with ab220031 at 1/500 dilution showing nuclear immunoreactivity in glandular cells and in the connective tissue.

  • Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4254] (ab220031)
    Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4254] (ab220031)

    Immunocytochemistry/ Immunofluorescence anaysis of RH-30 cells labeling METTL14 with ab220031 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

     
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate tissue labeling METTL14 with ab220031 at 1/500 dilution showing nuclear immunoreactivity in glandular cells and in smooth muscle cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human fallopian tube tissue labeling METTL14 with ab220031 at 1/500 dilution showing nuclear immunoreactivity in glandular cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling METTL14 with ab220031 at 1/500 dilution showing nuclear immunoreactivity in cells in seminiferous tubules.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4254] (ab220031)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human endometrium tissue labeling METTL14 with ab220031 at 1/500 dilution showing nuclear immunoreactivity in glandular cells and in stroma.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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