Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-METTL14 antibody [CL4252] (ab220030)

Key features and details

Mouse monoclonal [CL4252] to METTL14
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Human
Isotype: IgG2a

Product name

Anti-METTL14 antibody [CL4252]
See all METTL14 primary antibodies
Description

Mouse monoclonal [CL4252] to METTL14
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment (proprietary-tag) corresponding to Human METTL14 aa 1-100.
Database link: Q9HCE5

Run BLAST with Run BLAST with
Positive control
- Human testis, Human endometrium, Human prostate, Human cervix, Human cerebral cortex and Human duodenum tissues; A431 cell line lysate. ICC/IF: RH-30 cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL4252
Isotype

IgG2a
Research areas

Western blot - Anti-METTL14 antibody [CL4252] (ab220030)

Anti-METTL14 antibody [CL4252] (ab220030) at 1/500 dilution + A431 cell line lysate

Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human duodenum tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in the connective tissue.
Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4252] (ab220030)

Immunocytochemistry/ Immunofluorescence anaysis of RH-30 cells labeling METTL14 with ab220030 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cerebral cortex tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing moderate nuclear positivity in neurons.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervix tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in squamous ephitelium and connective tissue cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in smooth muscle.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human endometrium tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear immunoreactivity in glandular cells and in stroma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in cells in seminiferous tubes.

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