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Anti-METTL14 antibody [CL4252] (ab220030)

Key features and details

  • Mouse monoclonal [CL4252] to METTL14
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-METTL14 antibody [CL4252]
    See all METTL14 primary antibodies

  • Description

    Mouse monoclonal [CL4252] to METTL14

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment (proprietary-tag) corresponding to Human METTL14 aa 1-100.
    Database link: Q9HCE5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human testis, Human endometrium, Human prostate, Human cervix, Human cerebral cortex and Human duodenum tissues; A431 cell line lysate. ICC/IF: RH-30 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CL4252

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-METTL14 antibody [CL4252] (ab220030)
    Western blot - Anti-METTL14 antibody [CL4252] (ab220030)
    Anti-METTL14 antibody [CL4252] (ab220030) at 1/500 dilution + A431 cell line lysate

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human duodenum tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in the connective tissue.

  • Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4252] (ab220030)
    Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4252] (ab220030)

    Immunocytochemistry/ Immunofluorescence anaysis of RH-30 cells labeling METTL14 with ab220030 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

     
     
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cerebral cortex tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing moderate nuclear positivity in neurons.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervix tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in squamous ephitelium and connective tissue cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in smooth muscle.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human endometrium tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear immunoreactivity in glandular cells and in stroma.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (ab220030)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in cells in seminiferous tubes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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