Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Met (c-Met) antibody [EPR19554-110] (ab254252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19554-110] to Met (c-Met)
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Met (c-Met) antibody [EPR19554-110]
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [EPR19554-110] to Met (c-Met)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: B16-F10 whole cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Met (c-Met) antibody [EPR19554-110] (ab254252)
    Western blot - Anti-Met (c-Met) antibody [EPR19554-110] (ab254252)
    Anti-Met (c-Met) antibody [EPR19554-110] (ab254252) at 1/1000 dilution + B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 155 kDa



    The molecular weight observed is consistent with what has been described in the literature (PMID 8710887).

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Met (c-Met) antibody [EPR19554-110] (ab254252)
    Western blot - Anti-Met (c-Met) antibody [EPR19554-110] (ab254252)
    All lanes : Anti-Met (c-Met) antibody [EPR19554-110] (ab254252) at 1/1000 dilution

    Lane 1 : Untreated B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate
    Lane 2 : B16-F10 whole cell lysate treated with PNGase F

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 155 kDa
    Observed band size: 175-125 kDa
    why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

    c-Met is an N-linked glycosylated protein. After partial de-glycosylation, the sizes of the pro c-Met (175kDa) nd P125 c-Met (125kDa) are shifted to 160, 140kDa and 80kDa (PMID:23060267, PMID: 18187039).

  • Anti-Met (c-Met) antibody [EPR19554-110] (ab254252)
    Anti-Met (c-Met) antibody [EPR19554-110] (ab254252)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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