Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MelanA antibody [OTI3E2] (ab140503)

Key features and details

Mouse monoclonal [OTI3E2] to MelanA
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-MelanA antibody [OTI3E2]
See all MelanA primary antibodies
Description

Mouse monoclonal [OTI3E2] to MelanA
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	African green monkey
IHC-P	Human
WB	Dog Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human MelanA aa 1-118.
Sequence:
MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRR RNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNA PPAYEKLSAEQSPPPYSP

Run BLAST with Run BLAST with
Positive control
- WB: MDCK and HEK293T transfected with pCMV6-ENTRY MelanA cDNA cell lysate IHC-P: Human kidney and pancreas tissues ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY MelanA
General notes

The clone number has been updated from 3E2 to OTI3E2, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI3E2
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MelanA with ab140503 at 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [OTI3E2] (ab140503)

Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY MelanA, labelling MelanA with ab140503 at 1/100 dilution.
Western blot - Anti-MelanA antibody [OTI3E2] (ab140503)

Anti-MelanA antibody [OTI3E2] (ab140503) at 1/200 dilution + MDCK cell lysate at 10 µg

Predicted band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling MelanA with ab140503 at 1/150 dilution.
Western blot - Anti-MelanA antibody [OTI3E2] (ab140503)

All lanes : Anti-MelanA antibody [OTI3E2] (ab140503) at 1/4000 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MelanA

Lysates/proteins at 5 µg per lane.

Predicted band size: 13 kDa

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