Anti-MEK1 (phospho T386) antibody (ab277789)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal to MEK1 (phospho T386)
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-MEK1 (phospho T386) antibody
See all MEK1 primary antibodies -
Description
Rabbit recombinant multiclonal to MEK1 (phospho T386) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MEK1 (phospho T386).
Database link: Q02750 -
Positive control
- WB: HEK-293 whole cell extract, HEK-293 treated with EGF (100 ng/mL for 30 mins) whole cell extract. ICC: HeLa cells.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEK1 antibody (ab277789)All lanes : Anti-MEK1 (phospho T386) antibody (ab277789) at 2 µg/ml
Lane 1 : HEK-293 whole cell extract
Lane 2 : HEK-293 treated with EGF (100 ng/mL for 30 mins) whole cell extract
Secondary
All lanes : HRP conjugate Goat anti-Rabbit IgG (H+L) Superclonal, 0.4 µg/mL at 1/2500 dilution
Predicted band size: 43 kDa
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Immunocytochemistry - Anti-MEK1 antibody (ab277789)For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous Mek1 pT386 using Anti- Mek1 pT386 Recombinant Rabbit Multiclonal Antibody (ab277789, 2 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of Mek1 pT386 (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of Mek1 pT386 in dividing cells. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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Anti-MEK1 antibody (ab277789)